ESBB Innovative Technologies Webinar Series
The ESBB Members’ Webinar series are frequently organized throughout the year as a half-day to full-day exchange platform. During these webinars, state-of-the-art lectures on hot topics and ESBB working group results will be presented, and ESBB business reported. These webinars are open only to ESBB members in good standing on free registration.
ESBB Innovative Technologies Webinar Nr. I and II, 2020
Topic: Quality Control & Biomarker Detection by Nuclear Magnetic Resonance Spectroscopy
Nuclear magnetic resonance (NMR) spectroscopy is a versatile technique extensively used in the field of organic chemistry, structural biology and others. It exploits the magnetic properties of certain NMR active nuclei to give information about their local chemical environment (structure), dynamics, and about molecular size, concentration, and molecular interactions.
With these features, NMR has also developed into one of the major instrumental techniques for metabolic analysis of body fluids and tissues. While measuring only a relatively small portion of the human metabolome, NMR allows the simultaneous detection and identification of diverse metabolites in a non-destructive and high throughput manner, is intrinsically quantitative, highly reproducible, and requires only minimal preparation effort. Proton NMR-based metabolic phenotyping of body fluids and tissues provides important prognostic and diagnostic information and permits monitoring of disease progression. Moreover, metabolic screening by NMR can also be used to investigate the quality of input samples. It has been shown that metabolite concentrations are excellent quality markers reflecting the pre-analytical handling of a given biospecimen. Importantly, quality control data and analytical information are gained in the same experiment requiring less than 15 min of measurement time. From a biobankers point of view, both substantially increase the value of a sample. Furthermore, if spectra are acquired and analyzed under identical experimental conditions and standard operating procedures, the high reproducibility of NMR allows straightforward integration into large scale multicenter studies.
In the first part of this webinar series, the principles of 1H NMR are explained. Advantages and limitations of the technique are discussed and the NMR approach to metabolomics is introduced. The second part covers Bruker’s In Vitro Diagnostic research (IVDr) platform, including the instrumental set-up, SOPs, QC and the benefits of its usage across large-scale metabolic phenotyping studies.
Webinar I: Basics 1H NHR Spectroscopy for Metabolomics
Lead by: Dr. Friedemann Flügge, University of Lübeck, Germany
- NMR principles and the main NMR observables
o What is detected in an NMR experiment?
o Field strength and operating frequency
o Free Induction Decay, frequency, signal intensity and signal line width
o Standardization and chemical shift
- NMR strengths and weaknesses
o Detection limits
o Preparation effort
o Time effort
o Detected metabolites
o NMR experiments
o Data processing
o Targeted vs. untargeted analysis
o NMR metabolomics in routine analytics
o Pre-analytics and QC
Webinar II: NMR in Vitro Diagnostics Research Platform
Lead by: Dr. Roland Leiminger, Bruker BioSpin GmbH, Germany
o What does IVDr stand for?
o Equipment set-up and standards
o Pros, cons and limitations
o Costs, quality, reproducibility, transferability and reusability
- Standard Operating Procedures
o Implementation of SOPs
o SOP tools for different routine analytics
o Push Button technology
- Biobank Quality Control tool
o Quality parameters
o Limit of detection
o Metabolite information
o Lipoprotein subclass information
o Reproducibility and transferability
o ICPN phenome center network
o The role of biobanks
o Legal considerations
ESBB Innovative Technologies Webinar Nr. III and IV, 2020
Topic: more information coming soon
ESBB Innovative Technologies Webinar Nr. V and VI, 2020
Topic: more information coming soon